Feighny R, Burrous J, McCown J, Hoke C, Putnak R
Department of Virus Diseases, Walter Reed Army Institute of Research, Washington, DC.
Am J Trop Med Hyg. 1992 Oct;47(4):405-12. doi: 10.4269/ajtmh.1992.47.405.
Both the envelope structural protein and the non-structural NS1 protein have been purified from the flavivirus dengue-2 by high-pressure liquid chromatography. These purified proteins maintain their reactivity with monoclonal antibodies. When tested in mice, the envelope protein elicited neutralizing antibodies and partially protected the animals against a lethal viral challenge. The mice responded to the non-structural protein by producing antibodies; however, these antibodies were not neutralizing and the mice were not protected.
包膜结构蛋白和非结构NS1蛋白均已通过高压液相色谱法从黄病毒登革热2型中纯化出来。这些纯化后的蛋白保持了与单克隆抗体的反应活性。在小鼠实验中，包膜蛋白引发了中和抗体，并部分保护动物免受致命的病毒攻击。小鼠对非结构蛋白产生了抗体反应；然而，这些抗体没有中和作用，小鼠也未得到保护。
