Beach R F, Cordon-Rosales C, Molina E, Wirtz R A
Medical Entomology Research and Training Unit/Guatemala.
Am J Trop Med Hyg. 1992 Oct;47(4):478-83. doi: 10.4269/ajtmh.1992.47.478.
We have verified for specimens of Anopheles albimanus that an enzyme-linked immunosorbent assay (ELISA) used to assess Plasmodium vivax and P. falciparum sporozoite antigen rates gives results comparable to the salivary gland dissection method for estimating sporozoite rates. For 14,150 adults of An. albimanus, captured at five locations in Guatemala, we report sporozoite antigen rates of 0.03-0.57%, which correlate with the malaria prevalences at the study sites. We also present data that suggest that specimens of An. albimanus for the ELISA can be obtained more efficiently by cattle corral collections than by the human bait capture method.
我们已针对白纹按蚊样本证实，用于评估间日疟原虫和恶性疟原虫子孢子抗原率的酶联免疫吸附测定（ELISA）所给出的结果，与用于估计子孢子率的唾液腺解剖法相当。对于在危地马拉五个地点捕获的14150只白纹按蚊成虫，我们报告子孢子抗原率为0.03%-0.57%，这与研究地点的疟疾流行率相关。我们还提供了数据表明，通过牛栏收集法比人饵诱捕法能更有效地获取用于ELISA检测的白纹按蚊样本。
