Guasch R, Renau-Piqueras J, Guerri C
Instituto Investigaciones Citológicas, Valencia, Spain.
Alcohol Clin Exp Res. 1992 Oct;16(5):942-8. doi: 10.1111/j.1530-0277.1992.tb01897.x.
The effect of chronic ethanol consumption on labeled glycoprotein secretion and galactosyltransferase activity has been analyzed in cis- and trans-Golgi apparatus fractions isolated from rat liver. Rats were injected intraperitoneally with 3H-leucine, after different chase periods (30, 60, 180 min), and the radioactivity of the different subcellular fractions as well as of the isolated Golgi apparatus was measured. Chronic alcohol treatment induces an increase in liver weight as well as an enhancement of total liver protein. Ethanol treatment produces a significant accumulation of labeled proteins in isolated Golgi apparatus fractions after a 60- and 180-min chase. An accumulation of labeled proteins in the cytosolic fraction was observed only after 180 min. The alcohol treatment also induces a significant decrease in the activity of galactosyltransferase in both liver homogenate and Golgi apparatus fractions. These results suggest that an impairment of Golgi apparatus functions, including glycosylation and glycoprotein trafficking, could be one of the mechanisms involved in the accumulation of hepatic protein and thus in the pathogenesis of alcohol-induced injury in the liver of chronic ethanol-consuming animals.
在从大鼠肝脏分离出的顺式和反式高尔基体部分中，分析了长期乙醇摄入对标记糖蛋白分泌和半乳糖基转移酶活性的影响。在不同的追踪期（30、60、180分钟）后，给大鼠腹腔注射3H-亮氨酸，并测量不同亚细胞部分以及分离出的高尔基体的放射性。长期酒精处理会导致肝脏重量增加以及肝脏总蛋白增加。乙醇处理在追踪60分钟和180分钟后，会使分离出的高尔基体部分中标记蛋白显著积累。仅在180分钟后，才观察到胞质部分中标记蛋白的积累。酒精处理还会导致肝脏匀浆和高尔基体部分中半乳糖基转移酶的活性显著降低。这些结果表明，高尔基体功能的损害，包括糖基化和糖蛋白运输，可能是慢性乙醇摄入动物肝脏中蛋白质积累以及酒精性肝损伤发病机制所涉及的机制之一。
