血清蛋白在大鼠肝脏高尔基体中的转运。对高尔基体轻重组分的检测。
Passage of serum-destined proteins through the Golgi apparatus of rat liver. An examination of heavy and light Golgi fractions.
作者信息
Bergeron J J, Borts D, Cruz J
出版信息
J Cell Biol. 1978 Jan;76(1):87-97. doi: 10.1083/jcb.76.1.87.
Abstract
The participation of hepatic Golgi apparatus in the intracellular transport of blood-destined proteins has been analyzed using Golgi fractions enriched in cis and trans components of the Golgi apparatus. SDS-polyacrylamide gel electrophoresis of the liver Golgi fractions showed several proteins corresponding in relative proportions and mobilities with serum proteins. After a pulse injection of labeled leucine, the secretory content of the cis Golgi fraction was labeled earlier than the trans Golgi fraction. Taken together, the results show the participation of the liver Golgi apparatus in the secretion of most of the serum proteins and provide documentation for a sequential progression of secretory protein through the cis and trans components of the Golgi apparatus.
摘要
利用富含高尔基体顺式和反式组分的高尔基体组分，对肝脏高尔基体在血液定向蛋白的细胞内运输中的参与情况进行了分析。肝脏高尔基体组分的SDS-聚丙烯酰胺凝胶电泳显示，几种蛋白质在相对比例和迁移率上与血清蛋白相对应。在脉冲注射标记的亮氨酸后，顺式高尔基体组分的分泌内容物比反式高尔基体组分更早被标记。综合来看，结果表明肝脏高尔基体参与了大多数血清蛋白的分泌，并为分泌蛋白通过高尔基体的顺式和反式组分进行顺序转运提供了证据。
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