Hoffstetter-Kuhn S, Rösler T, Ehrat M, Widmer H M
Ciba-Geigy Ltd., Basel, Switzerland.
Anal Biochem. 1992 Nov 1;206(2):300-8. doi: 10.1016/0003-2697(92)90370-m.
The characterization and quantification of biomass is often time consuming and dependent on the cultivation media and gives no detailed information between cell size and shape and their productivity. By monitoring the bioprocess with steric sedimentation field-flow fractionation (Sd/StFFF) in combination with laser light scattering, not only cell growth, but also the variation of cell size and shape during the cultivation, can be observed. In this work, the feasibility of separating and characterizing cell populations by steric sedimentation field-flow fractionation is demonstrated by its application to three different yeast cultivation broths. For this purpose samples which were collected at different cultivation times were injected into an FFF system. Fractograms were obtained in less than 4 min. Due to the relatively high resolution of the method, a cell sample could be fractionated in several subpopulations differing in their size as well as in their number of buds.
生物质的表征和定量分析通常耗时且依赖于培养基，并且无法提供细胞大小、形状与其生产力之间的详细信息。通过将空间排阻沉降场流分离法（Sd/StFFF）与激光散射相结合来监测生物过程，不仅可以观察到细胞生长情况，还能观察到培养过程中细胞大小和形状的变化。在这项工作中，通过将空间排阻沉降场流分离法应用于三种不同的酵母培养液，证明了该方法用于分离和表征细胞群体的可行性。为此，将在不同培养时间收集的样品注入到场流分离系统中。不到4分钟即可获得分馏图。由于该方法具有相对较高的分辨率，细胞样品可被分离成几个大小不同且芽数量不同的亚群。
