Caldwell K D, Cheng Z Q, Hradecky P, Giddings J C
Cell Biophys. 1984 Dec;6(4):233-51. doi: 10.1007/BF02788630.
In this work, the feasibility of separating and characterizing cell populations by steric field-flow fractionation (steric FFF) is demonstrated by application to fixed human and avian red cells, fresh blood from several species, and viable HeLa cells. The basis for this work is established by means of a discussion of the role of steric FFF in the broad family of field-flow fractionation techniques. The behavior of steric FFF is then characterized by application to standard polystyrene latex beads and to fixed red blood cells. Studies of these standards and of the other cells noted under various conditions of field strength and flow velocity are used to improve the separation conditions and approach optimization. It is shown that the fixed human and avian red cells can be separated in a time of less than 15 min. In addition, it is shown that HeLa cells maintain their viability after passage through the separation channel.
在这项工作中，通过应用于固定的人类和禽类红细胞、来自多个物种的新鲜血液以及活的HeLa细胞，证明了利用空间场流分级法（steric FFF）分离和表征细胞群体的可行性。通过讨论空间场流分级法在广泛的场流分级技术家族中的作用，奠定了这项工作的基础。然后通过应用于标准聚苯乙烯乳胶珠和固定红细胞来表征空间场流分级法的行为。在不同场强和流速条件下对这些标准物以及其他细胞的研究被用于改善分离条件并接近优化。结果表明，固定的人类和禽类红细胞可在不到15分钟的时间内分离。此外，结果表明HeLa细胞在通过分离通道后仍保持其活力。
