Chang W B, Zhao Y B, Ci Y X, Hu L Y
Department of Chemistry, Peking University, Beijing, People's Republic of China.
Analyst. 1992 Aug;117(8):1377-8. doi: 10.1039/an9921701377.
A spectrofluorimetric method, involving alkaline degradation and formation of a magnesium complex, is described for the determination of tetracycline (TC) and anhydrotetracycline (ATC) in their mixed solution. Tetracycline is degraded and determined in alkaline solution. This treatment of ATC produces almost no fluorescence, but a fluorescent magnesium complex forms at pH 7.5. Several synthetic samples of TC and ATC, with TC:ATC ratios ranging from 50:1 to 1:50, were analysed. The recoveries of TC and ATC are about 71-76 and 61-63% in serum, respectively, and are all about 100% in urine.
本文描述了一种荧光分光光度法，该方法涉及碱性降解和镁配合物的形成，用于测定混合溶液中的四环素（TC）和脱水四环素（ATC）。四环素在碱性溶液中被降解并进行测定。这种处理对ATC几乎不产生荧光，但在pH 7.5时会形成荧光镁配合物。分析了几种TC和ATC的合成样品，其TC:ATC比例范围为50:1至1:50。TC和ATC在血清中的回收率分别约为71 - 76%和61 - 63%，在尿液中的回收率均约为100%。
