[白色念珠菌的快速鉴定：“Rapidec白色念珠菌”评估。对444株酵母菌株的研究]

[Rapid identification of Candida albicans: evaluation of "Rapidec albicans". Study of 444 yeast strains].

作者信息

Fricker H, Monget D, Lebeau B, Babolat M, Ambroise-Thomas P, Grillot R

机构信息

Département de parasitologie, mycologie médicale et moléculaire (DP3M), université J-Fourier-Grenoble I, URA CNRS 1344, CHU A-Michallon, France.

出版信息

Ann Biol Clin (Paris). 1992;50(2):103-6.

PMID:1443792

Abstract

Rapid identification of Candida albicans is of great importance as it is the most frequently isolated yeast pathogen. Rapidec albicans, a new 2-h micromethod, performs two fluorescent enzymatic activities: hexosaminidase and proline arylamidase. A total of 444 yeast strains (334 from type culture collections and 110 from recent clinical isolates) were tested. The sensitivity was 98.5% and the specificity 95.8%. When only considering the clinical strains, 47/47 Candida albicans were identified by Rapidec albicans (sensitivity 100%) but only 43/47 by the germ tube test (sensitivity 91.5%). The specificities of the two tests were respectively 98.2% and 100%. This new system is therefore very efficient for the routine diagnosis of Candida albicans in the clinical field. It is easier and quicker than the germ tube test.

摘要

白色念珠菌的快速鉴定至关重要，因为它是最常分离出的酵母病原体。Rapidec白色念珠菌检测法是一种新的两小时微量方法，可进行两种荧光酶活性检测：己糖胺酶和脯氨酸芳基酰胺酶。共检测了444株酵母菌株（334株来自标准菌种保藏中心，110株来自近期临床分离株）。其灵敏度为98.5%，特异性为95.8%。仅考虑临床菌株时，Rapidec白色念珠菌检测法鉴定出47/47株白色念珠菌（灵敏度100%），而芽管试验仅鉴定出43/47株（灵敏度91.5%）。两种检测方法的特异性分别为98.2%和100%。因此，这个新系统在临床领域对白色念珠菌的常规诊断非常有效。它比芽管试验更简便、快捷。