筛选从马来酸盐生产D-苹果酸盐的微生物。
Screening for microorganisms producing D-malate from maleate.
作者信息
van der Werf M J, van den Tweel W J, Hartmans S
机构信息
Department of Food Science, Wageningen Agricultural University, The Netherlands.
出版信息
Appl Environ Microbiol. 1992 Sep;58(9):2854-60. doi: 10.1128/aem.58.9.2854-2860.1992.
More than 300 microorganisms were screened for their ability to convert maleate into D-malate as a result of the action of maleate hydratase. Accumulation of fumarate during incubation of permeabilized cells with maleate was shown to be indicative of one of the two enzymes known to transform maleate. The ratio in which fumarate and malate accumulated could be used to estimate the enantiomeric composition of the malate formed. Many strains (n = 128) were found to be capable of converting maleate to D-malate with an enantiomeric purity of more than 97%. Pseudomonas pseudoalcaligenes NCIMB 9867 was selected for more detailed studies. Although this strain was not able to grow on maleate, permeabilized cells were able to degrade maleate to undetectable levels, with a concomitant formation of D-malate. The D-malate was formed with an enantiomeric purity of more than 99.97%.
对300多种微生物进行了筛选，以检测它们在苹果酸水合酶作用下将马来酸转化为D-苹果酸的能力。透化细胞与马来酸孵育期间富马酸的积累表明存在已知能转化马来酸的两种酶之一。富马酸和苹果酸积累的比例可用于估计所形成苹果酸的对映体组成。发现许多菌株（n = 128）能够将马来酸转化为对映体纯度超过97%的D-苹果酸。选择了假产碱假单胞菌NCIMB 9867进行更详细的研究。虽然该菌株不能在马来酸上生长，但透化细胞能够将马来酸降解到检测不到的水平，并同时形成D-苹果酸。所形成的D-苹果酸的对映体纯度超过99.97%。
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