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美洲大蠊的结合血浆蛋白。I. 正常状态。

The conjugated plasma proteins of the American cockroach. I. The normal state.

作者信息

SIAKOTOS A N

出版信息

J Gen Physiol. 1960 May;43(5):999-1013. doi: 10.1085/jgp.43.5.999.

DOI:10.1085/jgp.43.5.999
PMID:14446367
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2195056/
Abstract

Five protein fractions have been separated by paper electrophoresis from the plasma of the American cockroach. With the utilization of various staining procedures several of the plasma fractions were shown to be conjugated proteins. Two of these (fractions II and IV) are readily identifiable by their phospholipid, carbohydrate, and protein composition. A third conjugated protein, fraction III, is characterized by its high neutral lipid and sterol content. This lipoprotein is also sex-specific. Another fraction (I) contains neutral lipid, sterol, and protein but electrophoretically is more mobile than fraction III. Fraction V, the last and least mobile of the normally occurring proteins, possesses electrophoretic properties similar to human fibrinogen.

摘要

通过纸电泳已从美洲蟑螂的血浆中分离出五种蛋白质组分。利用各种染色程序表明,其中几种血浆组分是结合蛋白。其中两种(组分II和IV)可通过其磷脂、碳水化合物和蛋白质组成轻松识别。第三种结合蛋白,即组分III,其特点是中性脂质和甾醇含量高。这种脂蛋白也具有性别特异性。另一个组分(I)含有中性脂质、甾醇和蛋白质,但在电泳中比组分III移动性更强。组分V是正常存在的蛋白质中最后且移动性最小的,其电泳特性类似于人纤维蛋白原。

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引用本文的文献

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The conjugated plasma proteins of the American cockroach. II. Changes during the molting and clotting processes.美洲大蠊的结合血浆蛋白。II. 蜕皮和凝血过程中的变化。
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Immunology. 1971 Nov;21(5):817-28.
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The role of the haemocytes of Clitumnus extradentatus in haemolymph coagulation.克氏侧齿海蛞蝓血细胞在血淋巴凝固中的作用。
Cell Tissue Res. 1977 Sep 5;182(4):513-24. doi: 10.1007/BF00219833.

本文引用的文献

1
A method for localization and determination of serum lipids after electrophoretical separation on filter paper.一种在滤纸上进行电泳分离后定位和测定血清脂质的方法。
Scand J Clin Lab Invest. 1952;4(2):98-103. doi: 10.3109/00365515209060643.
2
The conjugated plasma proteins of the American cockroach. II. Changes during the molting and clotting processes.美洲大蠊的结合血浆蛋白。II. 蜕皮和凝血过程中的变化。
J Gen Physiol. 1960 May;43(5):1015-30. doi: 10.1085/jgp.43.5.1015.
3
Phospholipid detection in paper electrophoresis.纸电泳中的磷脂检测
Scand J Clin Lab Invest. 1955;7(3):277-8. doi: 10.3109/00365515509134525.
4
On the origin of the carbon in the induced synthesis beta-galactosidase in Escherichia coli.关于大肠杆菌中诱导合成β-半乳糖苷酶时碳的来源
J Bacteriol. 1954 Oct;68(4):419-29. doi: 10.1128/jb.68.4.419-429.1954.
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Electrophoresis of locust haemolymph.蝗虫血淋巴的电泳
Arch Int Physiol. 1953 Jun;61(3):413-6. doi: 10.3109/13813455309144333.