SIAKOTOS A N
J Gen Physiol. 1960 May;43(5):999-1013. doi: 10.1085/jgp.43.5.999.
Five protein fractions have been separated by paper electrophoresis from the plasma of the American cockroach. With the utilization of various staining procedures several of the plasma fractions were shown to be conjugated proteins. Two of these (fractions II and IV) are readily identifiable by their phospholipid, carbohydrate, and protein composition. A third conjugated protein, fraction III, is characterized by its high neutral lipid and sterol content. This lipoprotein is also sex-specific. Another fraction (I) contains neutral lipid, sterol, and protein but electrophoretically is more mobile than fraction III. Fraction V, the last and least mobile of the normally occurring proteins, possesses electrophoretic properties similar to human fibrinogen.
通过纸电泳已从美洲蟑螂的血浆中分离出五种蛋白质组分。利用各种染色程序表明,其中几种血浆组分是结合蛋白。其中两种(组分II和IV)可通过其磷脂、碳水化合物和蛋白质组成轻松识别。第三种结合蛋白,即组分III,其特点是中性脂质和甾醇含量高。这种脂蛋白也具有性别特异性。另一个组分(I)含有中性脂质、甾醇和蛋白质,但在电泳中比组分III移动性更强。组分V是正常存在的蛋白质中最后且移动性最小的,其电泳特性类似于人纤维蛋白原。