Ando T, Homma R, Ino Y, Ito G, Miyahara A, Yamakawa H, Iwaki M, Okumura Y, Suko M, Haida M
Section of Allergology, Research Laboratories, Torii & Co., Ltd.
Arerugi. 1992 Jun;41(6):704-7.
A trypsin-like protease was purified from mite (Dermatophagoides farinae) fecal extract. In SDS-PAGE, the mite trypsin-like protease showed a single band at 34 kD. The purified trypsin-like protease possessed potent allergenic activity. Both the twenty N-terminal amino acid sequence and the amino acid composition of the purified protease were very similar to those of Der f III. These data strongly suggest that the trypsin-like protease in the mite is a Der f III allergen.
从螨（粉尘螨）粪便提取物中纯化出一种类胰蛋白酶。在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）中，螨类胰蛋白酶呈现出一条位于34 kD的单条带。纯化后的类胰蛋白酶具有很强的致敏活性。纯化蛋白酶的N端二十个氨基酸序列和氨基酸组成均与Der f III的非常相似。这些数据有力地表明，螨中的类胰蛋白酶是一种Der f III变应原。
