Pryor W A, Wang K, Bermúdez E
Biodynamics Institute, Louisiana State University, Baton Rouge 70803-1800.
Biochem Biophys Res Commun. 1992 Oct 30;188(2):618-23. doi: 10.1016/0006-291x(92)91101-u.
Cholesterol, 1, which is present in both the lung lining fluid and cell membranes of lung tissue, reacts with ozone in aqueous systems to give 3 beta-hydroxy-5-oxo-5,6-secocholestan-6-al (2) as the major product. Reaction of 2 with 2,4-dinitrophenyl hydrazine (DNPH) in aqueous solutions, liposomes or lung extracts affords the anti and syn DNPH derivatives of 2 (3b and 3c) and of the rearrangement product 3,5-dihydroxy-B-norcholestane-6-carboxaldehyde (3a). These derivatives also are detected in lung tissue extracts from rats exposed to 1.3 ppm ozone for 12 hr.
胆固醇（1）存在于肺衬液和肺组织的细胞膜中，在水体系中与臭氧反应，生成主要产物3β-羟基-5-氧代-5,6-开环胆甾烷-6-醛（2）。2与2,4-二硝基苯肼（DNPH）在水溶液、脂质体或肺提取物中反应，生成2（3b和3c）以及重排产物3,5-二羟基-β-降胆甾烷-6-甲醛（3a）的反式和顺式DNPH衍生物。在暴露于1.3 ppm臭氧12小时的大鼠的肺组织提取物中也检测到了这些衍生物。
