Hiraoka K, Sasaguri Y, Komiya S, Inoue A, Morimatsu M
Department of Pathology, Kurume University School of Medicine, Japan.
Biochem Int. 1992 Sep;27(6):1083-91.
We investigated the effects of platelet-derived growth factor (PDGF), epidermal growth factor (EGF), and insulin on the cell proliferation of and the production of matrix metalloproteinases (MMPs) by rheumatoid synovial fibroblasts in order to determine the role of these agents in rheumatoid arthritis. PDGF stimulated rheumatoid synovial fibroblasts to increase DNA synthesis and the production of precursor forms of MMP-1 of M(r) = 53,000 and -3 of M(r) = 57,000. EGF and insulin also increased DNA synthesis and the production of these enzymes, but the amount of DNA or MMPs was smaller than that induced by PDGF. Since the production of matrix macromolecules and their degradation is essential for the remodelling of synovial tissue in rheumatoid arthritis, these data suggest that the production of MMP-1 and-3 by rheumatoid synovial fibroblasts in relation to cell proliferation plays an important role in the pathological process of rheumatoid arthritis.
我们研究了血小板衍生生长因子（PDGF）、表皮生长因子（EGF）和胰岛素对类风湿性滑膜成纤维细胞增殖及基质金属蛋白酶（MMPs）产生的影响，以确定这些因子在类风湿性关节炎中的作用。PDGF刺激类风湿性滑膜成纤维细胞增加DNA合成以及分子量为53,000的MMP-1和分子量为57,000的MMP-3前体形式的产生。EGF和胰岛素也增加了DNA合成以及这些酶的产生，但DNA或MMPs的量比PDGF诱导的量要少。由于基质大分子的产生及其降解对于类风湿性关节炎滑膜组织的重塑至关重要，这些数据表明，类风湿性滑膜成纤维细胞产生的MMP-1和MMP-3与细胞增殖相关，在类风湿性关节炎的病理过程中起重要作用。
