Wernicke D, Seyfert C, Hinzmann B, Gromnica-Ihle E
Max Delbrück Center for Molecular Medicine, Berlin-Buch, Germany.
J Rheumatol. 1996 Apr;23(4):590-5.
To analyze synovial membrane of patients with rheumatoid arthritis (RA) for the expression of unknown matrix metalloproteinases (MMP).
Degenerate oligonucleotides corresponding to highly conserved regions of the MMP gene family and the rapid amplification of cDNA ends (RACE) method have been used to search for new members of this gene family. MMP gene expression has been characterized by Northern blot analysis.
We cloned a MMP cDNA from the synovial membrane that is completely identical to the recently published collagenase 3 cDNA derived from a human breast cancer cDNA library (Freije, et al: J Biol Chem 1994;269:16766-73). Collagenase 3 is expressed in parallel with interstitial collagenase and stromelysin 1 in RA and osteoarthritis (OA). Collagenase 3 gene expression was not detected in several normal human tissues.
The expression of collagenase 3 in the synovial membrane in RA and OA suggests its involvement in articular tissue degradation.
分析类风湿关节炎（RA）患者滑膜中未知基质金属蛋白酶（MMP）的表达情况。
利用与MMP基因家族高度保守区域相对应的简并寡核苷酸及cDNA末端快速扩增（RACE）方法来寻找该基因家族的新成员。通过Northern印迹分析对MMP基因表达进行了表征。
我们从滑膜中克隆出一个MMP cDNA，它与最近发表的源自人乳腺癌cDNA文库的胶原酶3 cDNA完全相同（弗雷耶等人：《生物化学杂志》1994年；269:16766 - 73）。在RA和骨关节炎（OA）中，胶原酶3与间质胶原酶和基质溶解素1平行表达。在几种正常人体组织中未检测到胶原酶3基因表达。
RA和OA滑膜中胶原酶3的表达表明其参与关节组织降解。
