Jackson R J, Stewart H B, Sachs G
Cancer. 1977 Nov;40(5 Suppl):2487-96. doi: 10.1002/1097-0142(197711)40:5+<2487::aid-cncr2820400915>3.0.co;2-u.
Plasma membrane fractions from normal colon cells and a transplantable colon adenocarcinoma were isolated and purified by differential and zonal density centrifugation. Enrichment of normal and adenocarcinoma plasma membranes was found in zonal fractions I and II (ZI and ZII) following centrifugation in an 18--50% sucrose gradient. The distribution of various marker enzymes in normal colon preparations suggested an apical origin for the membranes obtained in zonal fraction I while zonal fraction II appeared to contain basal-lateral membrane fragments. Enzymatic analysis of the plasma membrane derived from the colon tumor indicated that these fractions possess a more uniform distribution of Na-K+ ATPase perhaps reflecting a dedifferentiated state. The plasma membrane fractions isolated should prove useful for investigation of transport and other properties of vesicles derived from malignant and normal colon cells.
通过差速离心和区带密度离心，从正常结肠细胞和一种可移植的结肠腺癌中分离并纯化了质膜组分。在18%-50%的蔗糖梯度中离心后，正常和腺癌质膜在区带组分I和II（ZI和ZII）中得到富集。正常结肠制剂中各种标记酶的分布表明，区带组分I中获得的膜起源于顶端，而区带组分II似乎包含基底外侧膜片段。对结肠肿瘤来源的质膜进行酶分析表明，这些组分中钠钾ATP酶的分布更为均匀，这可能反映了一种去分化状态。分离得到的质膜组分应有助于研究源自恶性和正常结肠细胞的囊泡的转运及其他特性。
