Røger M, Huitfeldt H S, Hovig T
Institute of Pathology, Rikshospitalet, Oslo, Norway.
APMIS. 1992 Oct;100(10):922-9. doi: 10.1111/j.1699-0463.1992.tb04020.x.
A detailed morphometric study of normal human blood platelets is described. The purpose has been to evaluate the morphological characteristics of platelets exposed to minimal handling procedures in order to obtain an optimal basis for the appraisal of platelets in disease. Blood from 10 healthy volunteers was collected directly into buffered glutaraldehyde and processed for electron microscopy. This platelet fixation procedure resulted in excellent preservation of resting platelet ultrastructure with one exception: the dense bodies. Compared to platelets fixed following washing procedures, our directly fixed platelets comprised fewer pseudopods and contained more glycogen. An unexpected feature of the open canalicular system was the apparent release of blisters interpreted as microvesicles. Employing a computerized image analyzer, 300 of the platelets were examined morphologically. The morphometric data thus obtained were analyzed statistically, resulting in a set of standard values for morphological characteristics of human platelets which we have found useful in subsequent evaluations of platelet morphology in disease. Significant inter-individual variance was, however, detected in two instances, in the section area of the alpha granules, as well as the area fraction of platelet sections occupied by channels of the open canalicular system (OCS). This should be taken into consideration when appraising platelet ultrastructure in health and disease.
本文描述了一项对正常人血小板的详细形态计量学研究。目的是评估经过最少处理程序的血小板的形态特征，以便为疾病中血小板的评估获得最佳依据。从10名健康志愿者采集的血液直接收集到缓冲戊二醛中，并进行电子显微镜处理。这种血小板固定程序能很好地保存静息血小板超微结构，但有一个例外：致密体。与经洗涤程序固定的血小板相比，我们直接固定的血小板伪足较少，糖原较多。开放小管系统一个意外的特征是明显释放出被解释为微泡的水泡。使用计算机图像分析仪对300个血小板进行形态学检查。对由此获得的形态计量数据进行统计分析，得出一组人血小板形态特征的标准值，我们发现这些值在随后疾病中血小板形态的评估中很有用。然而，在两个实例中检测到显著的个体间差异，即α颗粒的切片面积以及开放小管系统（OCS）通道所占血小板切片的面积分数。在评估健康和疾病状态下的血小板超微结构时应考虑到这一点。
