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[从烧伤病房分离的铜绿假单胞菌的质粒指纹图谱技术分析]

[Plasmid fingerprinting technique analysis of Pseudomonas aeruginosa isolated from a burn unit].

作者信息

Jia C G

机构信息

Fourth Military Medical University, Xian.

出版信息

Zhonghua Zheng Xing Shao Shang Wai Ke Za Zhi. 1992 Jun;8(2):139-40, 167.

PMID:1446293
Abstract

We used plasmid fingerprinting technique to analyse 50 strains of Pseudomonas aeruginosa isolated from our burn unit and 15 strains of Pseudomonas aeruginosa from other wards. The results revealed that the carrier rate of R-plasmid was 100%. All 50 strains of Pseudomonas aeruginosa from burn units contained one plasmid with approximate molecular weight of 55 kb. Among 15 strains of Pseudomonas aeruginosa from other wards, 10 strains contained one plasmid with the same molecular weight. However, 3 strains were found to possess an additional 12.5-kd plasmid and 2 strains contained 77-kb plasmid. This proves that R-plasmid carrying Pseudomonas aeruginosa is prevalent in the burn unit, and R-plasmid is disseminated in the hospital.

摘要

我们采用质粒指纹图谱技术,对从我院烧伤科分离出的50株铜绿假单胞菌以及从其他科室分离出的15株铜绿假单胞菌进行了分析。结果显示,R质粒的携带率为100%。烧伤科的所有50株铜绿假单胞菌均含有一个分子量约为55 kb的质粒。在其他科室的15株铜绿假单胞菌中,10株含有相同分子量的一个质粒。然而,发现3株还带有一个额外的12.5-kd质粒,2株含有77-kb质粒。这证明携带R质粒的铜绿假单胞菌在烧伤科很普遍,且R质粒在医院内传播。

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