Pradella S, Pletschette M, Mantey-Stiers F, Bautsch W
Institute of Medical Microbiology, Hannover Medical School, Germany.
Eur J Clin Microbiol Infect Dis. 1994 Feb;13(2):122-8. doi: 10.1007/BF01982184.
The colonization of hospitalized patients by Pseudomonas aeruginosa at an intensive care unit for burn victims was studied over a one-year period. A total of 231 isolates from 12 patients were analyzed by macrorestriction analysis. The results revealed that most patients were infected with only one to three different strains. In several patients Pseudomonas aeruginosa isolates of the same clonal lineage exhibited considerable differences in their macrorestriction fragment pattern. Digestion with further restriction enzymes, however, allowed differentiation between clonal variants due to a high genetic drift and superinfection with a different Pseudomonas aeruginosa strain. Isolates of the same clonal lineage could be isolated from several patients as well as from the patients' environment. Notably, Pseudomonas aeruginosa could be isolated from sedimentation plates. Thus, patients may have been extensively cross-infected on the ward. These data underline the importance of strict infection control measures and of regular surveillance for Pseudomonas aeruginosa by an appropriate typing method, i.e. one that can differentiate strains with high genomic variability.
在一年的时间里，对一家烧伤患者重症监护病房中铜绿假单胞菌在住院患者中的定植情况进行了研究。通过宏观限制性分析对来自12名患者的总共231株分离菌进行了分析。结果显示，大多数患者仅感染了一至三种不同的菌株。在几名患者中，同一克隆谱系的铜绿假单胞菌分离株在其宏观限制性片段模式上表现出相当大的差异。然而，用其他限制性酶进行消化后，由于存在高度的基因漂移以及被不同的铜绿假单胞菌菌株重叠感染，从而能够区分克隆变体。同一克隆谱系的分离株既可以从几名患者中分离得到，也可以从患者的环境中分离得到。值得注意的是，在沉降平板上也能分离出铜绿假单胞菌。因此，患者在病房中可能受到了广泛的交叉感染。这些数据强调了严格的感染控制措施以及通过适当的分型方法（即一种能够区分具有高基因组变异性菌株的方法）对铜绿假单胞菌进行定期监测的重要性。
