Inoue K, Matsumoto H, Koyama C, Shibata K, Nakazato Y, Ito A
Department of Morphology, Gunma University School of Medicine, Maebashi, Japan.
Endocrinology. 1992 Dec;131(6):3110-6. doi: 10.1210/endo.131.6.1446645.
An isologously transplantable mouse thyrotropic pituitary tumor (TtTb) that had been induced by radiothyroidectomy was found to form lobules composed of parenchymal glandular cell elements. The surfaces of these lobules were covered by stellate cells bearing long processes that gave a strong immunopositive reaction with a specific antiserum against glial fibrillary acidic protein (GFAP). GFAP was also positive in pituitary folliculo-stellate cells (FS cells). Since in the anterior pituitary gland, no other cellular component showing positive staining with anti-GFAP antibody was observed, it seems probable that the GFAP-positive cells in the thyrotropic tumor are related to the FS cells in the anterior pituitary. Upon cultivation of the thyrotropic tumor, cells with long processes appeared. These cells showed strong staining for GFAP and were dependent on basic fibroblast growth factor for cell growth. By repeated passage, the GFAP-positive cells became a stably growing immortal cell line, which was tentatively named TtT/GF. Since the characteristics of TtT/GF were similar to those of FS cells in the anterior pituitary, i.e. the presence of many lysosomes and numerous intermediate filaments in the cytoplasm, phagocytic activity, follicle formation, and GFAP and S-100 protein positivity, we suggest that TtT/GF cells are derived from pituitary FS cells.
一种经放射性甲状腺切除诱导产生的可同基因移植的小鼠促甲状腺垂体瘤（TtTb），被发现形成了由实质腺细胞成分组成的小叶。这些小叶的表面覆盖着带有长突起的星状细胞，这些细胞与抗胶质纤维酸性蛋白（GFAP）的特异性抗血清发生强烈的免疫阳性反应。GFAP在垂体滤泡星状细胞（FS细胞）中也呈阳性。由于在前叶垂体中，未观察到其他细胞成分对抗GFAP抗体呈阳性染色，因此促甲状腺肿瘤中GFAP阳性细胞似乎与前叶垂体中的FS细胞有关。在培养促甲状腺肿瘤时，出现了带有长突起的细胞。这些细胞对GFAP呈强染色，并且细胞生长依赖于碱性成纤维细胞生长因子。通过反复传代，GFAP阳性细胞成为稳定生长的永生细胞系，暂命名为TtT/GF。由于TtT/GF的特征与前叶垂体中FS细胞的特征相似，即细胞质中存在许多溶酶体和大量中间丝、吞噬活性、滤泡形成以及GFAP和S-100蛋白阳性，我们认为TtT/GF细胞来源于垂体FS细胞。
