Crook M, Machin S, Crawford N
Department of Biochemistry and Cell Biology, Hunterian Institute, Royal College of Surgeons, London, U.K.
Eur J Haematol. 1992 Sep;49(3):128-32. doi: 10.1111/j.1600-0609.1992.tb00916.x.
We have previously reported, for platelets from patients with idiopathic thrombocytopenic purpura, that there was in increased electrophoretic mobility that was related to their increased sialic acid content. Heterogeneity in the circulating platelet pool of patients with essential thrombocythaemia (platelet counts 600-1000 x 10(9)/l) has also been investigated by preparative continuous flow electrophoresis (CFE). The surface charge-dependent separation profiles of all the patients' platelets showed marked anodal shifts compared with the profiles of platelets from age- and sex-matched control subjects separated under identical conditions. The increase above normal in the net electronegativity of the patients' platelets could not be accounted for by differences in surface neuraminidase-labile or total platelet sialic acid content.
我们之前曾报道，对于特发性血小板减少性紫癜患者的血小板，其电泳迁移率增加，这与唾液酸含量增加有关。真性红细胞增多症患者（血小板计数为600 - 1000×10⁹/L）循环血小板池的异质性也已通过制备性连续流动电泳（CFE）进行了研究。与在相同条件下分离的年龄和性别匹配的对照受试者的血小板图谱相比，所有患者血小板的表面电荷依赖性分离图谱均显示出明显的阳极迁移。患者血小板净负电性高于正常水平，这无法通过表面神经氨酸酶不稳定或总血小板唾液酸含量的差异来解释。
