Janeczko K
Department of Neuroanatomy, Jagellonian University, Kraków, Poland.
Folia Histochem Cytobiol. 1992;30(1):27-33.
After an unilateral injury of the cerebral hemisphere, 28 nice were injected with 3H thymidine at different intervals following the injury. Thereafter, the distribution of autographically labelled astrocytes expressing glial fibrillary protein (GFAP) and astrocyte-like cells expressing vimentin were recorded within the region of injury. Proliferative activity of the two cell types started at the same time, i.e. 24 h. after injury, reached its maximum on day 4, and returned to normal level after the 8th posttraumatic day. However, on day 4 the number of proliferating GFAP-positive astrocytes was about 50% higher than that of vimentin-positive cells. This was regarded as a proof of the concept that a significant number of astrocytes did not express vimentin during its mitotic cycle. Those facts were considered as an evidence against the hypothesis that a reactive astrocyte division induces a two-stage increase in the cytoskeletal proteins level with the elevated synthesis of vimentin preceding that of GFAP.
在大脑半球单侧损伤后，28只大鼠在损伤后的不同时间间隔注射3H胸腺嘧啶核苷。此后，记录损伤区域内表达胶质纤维酸性蛋白（GFAP）的放射性自显影标记星形胶质细胞和表达波形蛋白的星形胶质样细胞的分布。两种细胞类型的增殖活性在同一时间开始，即损伤后24小时，在第4天达到最大值，并在创伤后第8天恢复到正常水平。然而，在第4天，增殖的GFAP阳性星形胶质细胞数量比波形蛋白阳性细胞数量高约50%。这被视为一个概念的证据，即大量星形胶质细胞在其有丝分裂周期中不表达波形蛋白。这些事实被视为反对以下假设的证据：反应性星形胶质细胞分裂会导致细胞骨架蛋白水平的两阶段增加，波形蛋白的合成增加先于GFAP。
