Hyland K, Bottiglieri T
Baylor University Medical Center, Kimberly H. Courtwright and Joseph W. Summers Metabolic Disease Center, Dallas, TX 75246.
J Chromatogr. 1992 Aug 7;579(1):55-62. doi: 10.1016/0378-4347(92)80362-t.
Precolumn derivatization of amino acids with o-phthaldialdehyde followed by high-performance liquid chromatographic separation and fluorescence detection is used in many clinical and experimental laboratories for the measurement of primary amino acids. This technique was adapted for the measurement of homocysteine in plasma and cerebrospinal fluid (CSF) following alkylation of the free sulphydryl group with iodoacetate. The minimum detection limits are less than 1 microM in plasma and 80 nM in CSF. Within-day and between-day coefficients of variation for plasma and for CSF are less than 10%. Values for normal plasma homocysteine range from 6.04 to 16.2 microM and for CSF from 0.28 to 0.66 microM.
许多临床和实验实验室采用邻苯二甲醛对氨基酸进行柱前衍生，随后进行高效液相色谱分离和荧光检测，以测定一级氨基酸。在用碘乙酸酯对游离巯基进行烷基化后，该技术被用于测定血浆和脑脊液（CSF）中的同型半胱氨酸。血浆中的最低检测限低于1微摩尔/升，脑脊液中的最低检测限为80纳摩尔/升。血浆和脑脊液日内及日间变异系数均小于10%。正常血浆同型半胱氨酸值范围为6.04至16.2微摩尔/升，脑脊液为0.28至0.66微摩尔/升。
