柱前衍生化法用邻苯二甲醛快速测定人尿液中的同型半胱氨酸。
On-column derivatization with o-phthaldialdehyde for fast determination of homocysteine in human urine.
机构信息
Department of Environmental Chemistry, University of Łódź, 90-131 Łódź, Poland.
出版信息
Anal Bioanal Chem. 2010 Mar;396(6):2363-6. doi: 10.1007/s00216-010-3456-7. Epub 2010 Feb 2.
PMID:20127080
Abstract
A new assay for urinary homocysteine is described. The assay relies on an on-column derivatization with o-phthaldialdehyde, using a reversed-phase HPLC column and detection/quantification by fluorescence. The analysis time for reduced and total homocysteine, including sample work-up, was 5 and 13 min, respectively. Quantification limit was 25 nM.
摘要
介绍了一种新的尿同型半胱氨酸检测方法。该方法依赖于柱上与邻苯二醛的衍生化反应，使用反相 HPLC 柱，并通过荧光进行检测/定量。还原型和总同型半胱氨酸的分析时间分别为 5 分钟和 13 分钟，包括样品制备。定量限为 25 nM。
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