al-Hasani S, Hepnar C, Diedrich K, van der Ven H, Krebs D
Universitäts-Frauenklinik und Hebammenlehranstalt, Bonn, FRG.
Hum Reprod. 1992 Jun;7 Suppl 1:81-3. doi: 10.1093/humrep/7.suppl_1.81.
Rabbit embryos at the pronuclear stage (n = 480) were frozen using a mixture of propanediol and sucrose. The survival rate was 71% according to their morphological features shortly after thawing. The viability rate after 5 days of culture was 10% according to the number of embryos that reached the blastocyst stage, while the rate of formation of blastocysts from freshly collected and cultured pronuclear embryos was higher (59%). The implantation rate was higher (24%) if the frozen/thawed zygotes were directly transferred to foster mothers after 2-4 hours of culture, whereas with zygotes cultured for 24 hours (4 to 8-cell stages) the implantation rate was poor (4%). Both rates were low compared with the implantation rate (52%) achieved with freshly collected and directly transferred pronucleate embryos.
使用丙二醇和蔗糖的混合物对原核期兔胚胎（n = 480）进行冷冻。根据解冻后不久的形态特征，存活率为71%。根据发育到囊胚期的胚胎数量，培养5天后的活力率为10%，而新鲜采集并培养的原核胚胎形成囊胚的比率更高（59%）。如果冷冻/解冻后的合子在培养2 - 4小时后直接移植到代孕母兔体内，着床率较高（24%），而培养24小时（4至8细胞期）的合子着床率较低（4%）。与新鲜采集并直接移植的原核胚胎的着床率（52%）相比，这两个比率都较低。
