Ichikawa T
Department of Orthopaedic Surgery, School of Medicine, Keio University, Tokyo, Japan.
Nihon Seikeigeka Gakkai Zasshi. 1992 Sep;66(9):931-48.
A histological and histochemical study of the repairing process of skeletal muscle after transection and immediate suture was conducted using the gastrocnemius muscle of hamsters. The muscles were examined from one hour to twelve weeks after operation and were prepared as specimens for either transverse or longitudinal sections. During the first two days, necrotic fibers were scavenged by macrophages. Early regenerating fibers appeared at the extent of about 1-2 mm on both sides of the cut ends by the fourth day. After one week, myotubes identified as type 2C were abundant. By the fourth week regenerating fibers had grown longitudinally and bridged the injury site between the proximal and distal muscle fiber segments. After six weeks, they had almost matured histochemically. After eight weeks, there was substantial repair of the injury site with scarce fibrous tissue. This study has clearly demonstrated the histological and histochemical findings of repairing process of the skeletal muscle after transection.
利用仓鼠的腓肠肌，对骨骼肌横断并立即缝合后的修复过程进行了组织学和组织化学研究。在术后1小时至12周对肌肉进行检查，并制备横切或纵切标本。在头两天，坏死纤维被巨噬细胞清除。到第4天，早期再生纤维出现在切口两端两侧约1-2毫米的范围内。1周后，大量被鉴定为2C型的肌管出现。到第4周，再生纤维纵向生长并连接了近端和远端肌纤维段之间的损伤部位。6周后，它们在组织化学上几乎成熟。8周后，损伤部位得到实质性修复，纤维组织稀少。本研究清楚地展示了骨骼肌横断后修复过程的组织学和组织化学结果。
