Mizutani K
Nihon Seikeigeka Gakkai Zasshi. 1981 Dec;55(12):1673-91.
Histological and histochemical study was carried out to investigate disuse atrophy on skeletal muscle in adult albino rabbits. Untreated anterior tibial muscle and gastrocnemius muscle were studied. Three fiber types were recognized with myosin ATPase reaction, viz. type I, IIA and IIB fibers under a positive correlation with succinate dehydrogenase, phosphorylase, lactate dehydrogenase and NADH. TR stain. Statistical analysis in histogram of fiber diameter was also done. Soleus muscle mainly consisted of type I and type IIA fibers. As to developing skeletal muscle after birth in rabbits, especially in neonatal periods, type IIC fiber and also myotubes were demonstrated, with a gradual decrease in the process of growth. Fiber type ratio were examined in the anterior tibial and soleus muscle. In soleus muscle, type I fiber increased in numbers along with growth. Fibers, reacting with both ATPase pH 9.4 and SDH stain decreased in number. In the anterior tibial muscle, on the other hand, numbers of type I fiber were unremarkable, but all of the fibers were stained moderately with succinate dehydrogenase in neonatal periods, and fibers, with evident ATPase (pH 9.4) and SDH stain, decreased in numbers with growth. Instead, type IIB fibers appeared. Cast immobilization were applied on the legs of rabbits under three different positions, namely in so-called neutral, relaxed and stretched, in order to examine anterior tibial and gastrocnemius muscle respectively. In the neutral posture, three to four weeks after immobilization, decrease in size of type IIA fibers in the anterior tibial muscle was remarkable, whereas, in gastrocnemius muscle less than three weeks after immobilization type IIA and also type I fibers were involved predominantly. In gastrocnemius muscle under relaxed posture, type IIA and also type I fiber were decreased in fiber diameter only two weeks after immobilization, whereas, under stretched posture only type IIA fibers were involved three to four weeks after immobilization. In the anterior tibial muscle under stretched posture, same results were not obtained as in the gastrocnemius muscle. Less significant change of fiber type ratio was recognized in the anterior tibial muscle after four weeks immobilization.
进行了组织学和组织化学研究，以调查成年白化兔骨骼肌的废用性萎缩。研究了未经处理的胫前肌和腓肠肌。通过肌球蛋白ATP酶反应识别出三种纤维类型，即I型、IIA型和IIB型纤维，它们与琥珀酸脱氢酶、磷酸化酶、乳酸脱氢酶和NADH呈正相关。TR染色。还对纤维直径直方图进行了统计分析。比目鱼肌主要由I型和IIA型纤维组成。至于兔子出生后尤其是新生儿期发育中的骨骼肌，发现了IIC型纤维和肌管，并且在生长过程中逐渐减少。检查了胫前肌和比目鱼肌的纤维类型比例。在比目鱼肌中，I型纤维数量随生长增加。与ATP酶pH 9.4和SDH染色均反应的纤维数量减少。另一方面，在胫前肌中，I型纤维数量不明显，但在新生儿期所有纤维均被琥珀酸脱氢酶中度染色，并且随着生长，具有明显ATP酶（pH 9.4）和SDH染色的纤维数量减少。取而代之的是出现了IIB型纤维。将兔子的腿部固定在三种不同位置，即所谓的中立位、松弛位和伸展位，以分别检查胫前肌和腓肠肌。在中立姿势下，固定三到四周后，胫前肌中IIA型纤维大小的减小很明显，而在腓肠肌中，固定不到三周时，IIA型和I型纤维主要受累。在松弛姿势下的腓肠肌中，固定仅两周后，IIA型和I型纤维的直径就减小了，而在伸展姿势下，固定三到四周后仅IIA型纤维受累。在伸展姿势下的胫前肌中，未获得与腓肠肌相同的结果。固定四周后，胫前肌中纤维类型比例的变化不太明显。
