MORSE S I
J Exp Med. 1962 Aug 1;116(2):229-45. doi: 10.1084/jem.116.2.229.
The cell walls of an 80/81 strain of Staphylococcus aureus (NYH-6) contain alanine, glycine, glutamic acid, lysine, muramic acid, glucosamine, and ribitol phosphate. 94 per cent of the phosphorus and 41 per cent of the glucosamine are removed by extraction of the cell walls with hot 5 per cent TCA, but significant amounts of the other constituents are not extracted by this procedure. The residue after hot TCA extraction (mucopeptide) is susceptible to lysozyme whereas the intact cell walls are resistant. Staphylococcus aureus cell walls are agglutinated by S. aureus antisera. Agglutination of the cell walls of one S. aureus strain is inhibited by absorption of antisera with cell walls of other S. aureus strains but not by absorption with S. albus cell walls. The ribitol teichoic acid can be isolated from cold TCA extracts of the cell walls. This compound consists almost entirely of ribitol phosphate and glucosamine. The isolated teichoic acid of strain NYH-6 is readily fixed to tanned sheep erythrocytes and these sensitized cells are agglutinated by S. aureus antisera. Cold TCA extracts of cell walls of other strains of S. aureus inhibit hemagglutination whereas extracts of S. albus walls do not. Studies on the inhibition of both hemagglutination and precipitation indicate that the antigenic determinant of S. aureus NYH-6 teichoic acid is beta-N-acetylglucosamine.
金黄色葡萄球菌（NYH - 6）80/81菌株的细胞壁含有丙氨酸、甘氨酸、谷氨酸、赖氨酸、胞壁酸、葡糖胺和磷酸核糖醇。用热的5%三氯乙酸（TCA）提取细胞壁时，94%的磷和41%的葡糖胺被去除，但该方法不能提取大量的其他成分。热TCA提取后的残渣（粘肽）对溶菌酶敏感，而完整的细胞壁则具有抗性。金黄色葡萄球菌细胞壁被金黄色葡萄球菌抗血清凝集。一种金黄色葡萄球菌菌株的细胞壁凝集可被其他金黄色葡萄球菌菌株的细胞壁吸收抗血清所抑制，但不能被白色葡萄球菌细胞壁吸收所抑制。磷酸核糖醇壁酸可从细胞壁的冷TCA提取物中分离出来。该化合物几乎完全由磷酸核糖醇和葡糖胺组成。分离出的NYH - 6菌株壁酸很容易固定在鞣酸处理的绵羊红细胞上，这些致敏细胞被金黄色葡萄球菌抗血清凝集。其他金黄色葡萄球菌菌株细胞壁的冷TCA提取物可抑制血凝，而白色葡萄球菌细胞壁提取物则不能。对血凝和沉淀抑制的研究表明，金黄色葡萄球菌NYH - 6壁酸的抗原决定簇是β - N - 乙酰葡糖胺。
