MURPHY W H, BULLIS C
J Bacteriol. 1962 Mar;83(3):641-8. doi: 10.1128/jb.83.3.641-648.1962.
Murphy, W. H. (The University of Michigan, Ann Arbor) and Cora Bullis. Effect of triiodothyronine on cells and on their response to infection by polioviruses. J. Bacteriol. 83:641-648. 1962.-An analysis was made of the effect of triiodothyronine (T3) at physiological (1 mug/ml) and maximal subliminal toxic levels (35 mug/ml) on HeLa-S3, HeLa-Gey, Chang-liver, and Maben cells, and on their response to infection by cytopathic and submoderate (noncytopathic) mutants of type 2 poliovirus. Assays of cell response to T3 alone, or in combination with the mutants of poliovirus, were made by conventional monolayer cell culture techniques, by study of the effect of T3 on plating efficiency of cells, and by study of its influence on colonies of cell variants. Cellular response to liminal doses of T3 was characterized by agglutination of cells and thickening of the cell membrane. Compact colonies of Chang-liver and Maben cells were the most sensitive to maximal subliminal amounts of T3. T3 in combination with cytopathic or submoderate (noncytopathic) mutants of poliovirus slightly increased the rate of destruction of cells susceptible to virus, but did not influence yield of virus from cell cultures. T3 at physiological or subliminal concentrations did not induce cytopathic response of cell cultures latently infected by submoderate poliovirus.
墨菲，W. H.（密歇根大学，安阿伯分校）和科拉·布利斯。三碘甲状腺原氨酸对细胞及其对脊髓灰质炎病毒感染反应的影响。《细菌学杂志》83:641 - 648。1962年。——分析了生理浓度（1微克/毫升）和最大阈下毒性浓度（35微克/毫升）的三碘甲状腺原氨酸（T3）对海拉 - S3细胞、海拉 - 盖伊细胞、张氏肝细胞和马本细胞的影响，以及它们对2型脊髓灰质炎病毒细胞病变和亚中等（非细胞病变）突变体感染的反应。通过传统的单层细胞培养技术、研究T3对细胞铺板效率的影响以及研究其对细胞变体集落的影响，来检测细胞对单独的T3或与脊髓灰质炎病毒突变体组合的反应。细胞对阈下剂量T3的反应特征为细胞凝集和细胞膜增厚。张氏肝细胞和马本细胞的紧密集落对最大阈下量的T3最为敏感。T3与脊髓灰质炎病毒的细胞病变或亚中等（非细胞病变）突变体组合，略微增加了对病毒敏感细胞的破坏速率，但不影响细胞培养物中病毒的产量。生理浓度或阈下浓度的T3不会诱导被亚中等脊髓灰质炎病毒潜伏感染的细胞培养物出现细胞病变反应。
