SCHERER W F, SYVERTON J T, GEY G O
J Exp Med. 1953 May;97(5):695-710. doi: 10.1084/jem.97.5.695.
The cells of a human epithelial cancer cultivated en masse have been shown to support the multiplication of all three types of poliomyelitis virus. These cells (strain HeLa of Gey) have been maintained in vitro since their derivation from an epidermoid carcinoma of the cervix in February, 1951. As the virus multiplied it caused in from 12 to 96 hours degeneration and destruction of the cancer cells. The specific destructive effect of the virus was prevented by adding homotypic antibody to the cultures but not by adding heterotypic antibodies. Methods for the preparation of large numbers of replicate cultures with suspensions of strain HeLa cells were described. The cells in suspension were readily quantitated by direct counts in a hemocytometer. A synthetic solution that maintains cellular viability was employed for viral propagation. The experimental results demonstrate the usefulness of strain HeLa cells for (a) the quantitation of poliomyelitis virus, (b) the measurement of poliomyelitis antibodies, and (c) the production of virus.
大量培养的人上皮癌细胞已被证明能支持所有三种脊髓灰质炎病毒的繁殖。这些细胞（盖伊的海拉细胞系）自1951年2月从子宫颈表皮样癌分离出来后一直在体外培养。随着病毒的繁殖，在12到96小时内它导致癌细胞变性和破坏。向培养物中加入同型抗体可防止病毒的特异性破坏作用，但加入异型抗体则不能。描述了用海拉细胞系悬液制备大量重复培养物的方法。悬浮液中的细胞通过血细胞计数器直接计数很容易进行定量。一种维持细胞活力的合成溶液用于病毒繁殖。实验结果证明海拉细胞系可用于：（a）脊髓灰质炎病毒的定量；（b）脊髓灰质炎抗体的测定；（c）病毒的生产。
