Shimabukuro T, Naito K
Department of Urology, Yamaguchi University School of Medicine.
Nihon Rinsho. 1992 Oct;50(10):2391-4.
It is very important to determine the biological characteristics of individual cancers, not only in order to evaluate malignant potentials but also to decide on the most effective anticancer therapy. A method which combines BrdU labeling and flow cytometric bromodeoxyuridine (BrdU)/DNA analysis makes it possible to plot the DNA synthetic rate of cells against their DNA content. Hence, this method yields more useful information than the previous method of flow cytometric DNA analysis. When this method is performed correctly, it can be also applied to fresh solid tumors. In this paper, we will describe the results of our preliminary investigation and discuss its clinical significance.
确定个体癌症的生物学特性非常重要，这不仅是为了评估恶性潜能，也是为了确定最有效的抗癌治疗方法。一种将BrdU标记与流式细胞术溴脱氧尿苷（BrdU）/DNA分析相结合的方法，能够绘制细胞的DNA合成率与其DNA含量的关系图。因此，该方法比以前的流式细胞术DNA分析方法能产生更有用的信息。如果正确执行该方法，它也可应用于新鲜实体瘤。在本文中，我们将描述初步研究的结果并讨论其临床意义。
