Shimabukuro T, Yoshihiro S, Matsuyama H, Yamamoto N, Sakatoku J
Department of Urology, Yamaguchi University School of Medicine.
Hinyokika Kiyo. 1989 Oct;35(10):1653-7.
We performed the simultaneous flow cytometric bromodeoxyuridine (BrdU)/DNA analysis in combination with in vitro BrdU labeling method using seven human urogenital tumors. The DNA content and incorporated BrdU content of each tumor cell was analyzed quantitatively using this flow cytometric method. The cell kinetics of each cell line of heterogeneous tumor could be analyzed by this combined method. In the near future, by establishing a procedure decreasing non-specific staining of cells, the development of this flow cytometric two-color analysis in clinical fields is expected.
我们使用七种人类泌尿生殖系统肿瘤,将流式细胞术溴脱氧尿苷(BrdU)/DNA分析与体外BrdU标记方法相结合进行了研究。采用这种流式细胞术方法对每个肿瘤细胞的DNA含量和掺入的BrdU含量进行了定量分析。通过这种联合方法可以分析异质性肿瘤各细胞系的细胞动力学。在不久的将来,通过建立减少细胞非特异性染色的程序,有望在临床领域开发这种流式细胞术双色分析方法。