ORLANS E
Immunology. 1962 Mar;5(2):306-21.
Specific precipitates formed in 0.9 per cent and 8 per cent NaCl and the precipitates formed by raising the salt concentration of `0.9 per cent' supernatants to 8 per cent were measured quantitatively. With antisera to haemoglobin and myoglobin the antigen in the precipitates was also measured. Except for some very high antibody/antigen ratios found in some cases in antibody excess, these ratios were the same as those found with rabbit antibody, and did not depend on salt concentration. Non-precipitating antibody, prepared by serial absorption of antiserum with small portions of antigen, did not precipitate with antigen even in 8 per cent NaCl; it co-precipitated with homologous rabbit antiserum and delayed its flocculation, but produced no permanent inhibition. Rabbit antiserum to washed specific precipitates made from fowl antisera was used to confirm the presence of two globulins, one a macroglobulin, in the precipitates, and to study their different properties when free in whole serum and when combined with antigen.
对在0.9%和8%氯化钠中形成的特定沉淀物以及通过将“0.9%”上清液的盐浓度提高到8%而形成的沉淀物进行了定量测量。使用针对血红蛋白和肌红蛋白的抗血清，还对沉淀物中的抗原进行了测量。除了在某些抗体过量的情况下发现一些非常高的抗体/抗原比值外，这些比值与用兔抗体获得的比值相同，并且不依赖于盐浓度。通过用小部分抗原连续吸收抗血清制备的非沉淀抗体，即使在8%氯化钠中也不与抗原沉淀；它与同源兔抗血清共沉淀并延迟其絮凝，但没有产生永久性抑制。用针对由禽抗血清制成的洗涤后特定沉淀物的兔抗血清来确认沉淀物中存在两种球蛋白，一种是巨球蛋白，并研究它们在全血清中游离时以及与抗原结合时的不同特性。
