Meyer W J, Gauthier D W
Department of Obstetrics and Gynecology, University of Illinois, Chicago.
Obstet Gynecol. 1992 Dec;80(6):1017-9.
To evaluate the effects of time and storage temperature on amniotic fluid (AF) glucose concentration.
Amniocentesis was performed on 47 patients with preterm labor or preterm rupture of membranes at or before 34 weeks' estimated gestation and the AF was cultured for aerobic, anaerobic, Mycoplasma, and Ureaplasma organisms. Twenty-one samples were stored at 37C, 13 were kept at room temperature (22C), and 13 were frozen (-20C). Glucose concentration was measured at 0 and 12 hours on all AF samples and at 2, 4, and 6 hours on the unfrozen samples.
No significant change in AF glucose concentration occurred over time at any storage temperature in the 35 samples with negative cultures. In 12 AF samples with positive cultures, a significant decrease from baseline was noted at 2 and 4 hours in those kept at 37C and 22C (P = .016). Glucose concentration was unchanged in frozen samples regardless of the culture results. The initial AF glucose concentration was significantly lower in samples with positive than in those with negative cultures (P < .0001).
Amniotic fluid glucose concentration does not change over 12 hours in the absence of intra-amniotic infection.
评估时间和储存温度对羊水（AF）葡萄糖浓度的影响。
对47例孕周估计在34周及以前发生早产或胎膜早破的患者进行羊膜腔穿刺术，并对羊水进行需氧菌、厌氧菌、支原体和脲原体培养。21个样本储存在37℃，13个样本保存在室温（22℃），13个样本冷冻保存（-20℃）。对所有羊水样本在0小时和12小时时测量葡萄糖浓度，对未冷冻样本在2小时、4小时和6小时时测量葡萄糖浓度。
35个培养结果为阴性的样本在任何储存温度下，羊水葡萄糖浓度随时间均无显著变化。在12个培养结果为阳性的羊水样本中，保存在37℃和22℃的样本在2小时和4小时时较基线水平显著降低（P = 0.016）。无论培养结果如何，冷冻样本中的葡萄糖浓度均无变化。培养结果为阳性的样本中羊水初始葡萄糖浓度显著低于培养结果为阴性的样本（P < 0.0001）。
在无羊膜腔内感染的情况下，羊水葡萄糖浓度在12小时内无变化。
