PATTEE P A, BALDWIN J N
J Bacteriol. 1961 Dec;82(6):875-81. doi: 10.1128/jb.82.6.875-881.1961.
Pattee, P. A. (Ohio State University, Columbus) and J. N. Baldwin. Transduction of resistance to chlortetracycline and novobiocin in Staphylococcus aureus. J. Bacteriol. 82: 875-881. 1961.-Using phage 80 of the International Typing Series propagated on appropriate strains of Staphylococcus aureus, resistance to chlortetracycline and novobiocin and the capacity to produce penicillinase were transduced to a number of antibiotic-sensitive recipient strains of S. aureus. The frequency of transduction varied from 1 to 10 transductants per 10(7) phage particles employed. Lysogenization of the transductants by the transducing phage did not occur. Phages 29, 52A, 79, and 53 of the International Typing Series were also capable of transduction, while phages 42B and 81 were unable to participate under the conditions used. The recipient strains were either resistant or susceptible to lysis by the transducing phages, but this did not influence the transduction frequencies. Lysis of transductants susceptible to the transducing phages was inhibited by preparing the selective medium with brain heart infusion agar. Linked transductions were not detected. With few exceptions, all of the recipient strains were susceptible to lysis by phages of group I or phage 81 or both. Strains susceptible to lysis by phages of groups II and III, but resistant to lysis by phages of group I or phage 81, were not transduced.
帕蒂，P.A.（俄亥俄州立大学，哥伦布）和J.N.鲍德温。金黄色葡萄球菌对金霉素和新生霉素耐药性的转导。《细菌学杂志》82:875 - 881。1961年。——利用在合适的金黄色葡萄球菌菌株上繁殖的国际分型系列噬菌体80，将对金霉素和新生霉素的耐药性以及产生青霉素酶的能力转导到多个对多种抗生素敏感的金黄色葡萄球菌受体菌株中。转导频率为每10⁷个所用噬菌体颗粒产生1至10个转导子。转导噬菌体未使转导子发生溶原化。国际分型系列的噬菌体29、52A、79和53也能够进行转导，而噬菌体42B和81在所采用的条件下无法参与转导。受体菌株对转导噬菌体的裂解作用有的敏感，有的耐药，但这并不影响转导频率。通过用脑心浸液琼脂制备选择性培养基，可抑制对转导噬菌体敏感的转导子的裂解。未检测到连锁转导。除少数例外，所有受体菌株对I组噬菌体或噬菌体81或两者的裂解作用均敏感。对II组和III组噬菌体的裂解敏感但对I组噬菌体或噬菌体81的裂解耐药的菌株未被转导。
