Tsuda A, Tanaka M, Georgiev V, Emoto H
Department of Pharmacology, Kurume University School of Medicine, Japan.
Pharmacol Biochem Behav. 1992 Nov;43(3):729-32. doi: 10.1016/0091-3057(92)90401-z.
The effects of angiotensin II (ATII) administered intracerebroventricularly in male Wistar rats in doses of 0.1, 0.5, and 1.0 micrograms, as well as of ATII (1.0 micrograms) + saralasin (SAR, an analog ATII) (5.0 micrograms), on behavioral responses of the defensive burying paradigm were studied. ATII-treated animals displayed significantly less defensive burying behavior (less time spent in defensive burying and less frequent burying than in vehicle-treated rats) in a dose-dependent manner. SAR at a dose of 5 micrograms did not affect burying behavior significantly; it also did not modify the inhibition effects of ATII on behavioral responses of the defensive burying test. These results provide evidence that ATII can exert anxiolytic actions on central transmitter systems mediating conditioned fear-related behaviors (i.e., defensive burying). The present study suggests that the defensive burying animal model is a rather sensitive test fulfilling the pharmacological criteria of dose-dependent sensitivity for studying the central effects of neuropeptides (e.g., ATII).
研究了以0.1、0.5和1.0微克剂量脑室内注射血管紧张素II（ATII），以及ATII（1.0微克）+沙拉新（SAR，一种ATII类似物）（5.0微克）对雄性Wistar大鼠防御性埋埋范式行为反应的影响。经ATII处理的动物表现出明显较少的防御性埋埋行为（与赋形剂处理的大鼠相比，防御性埋埋花费的时间更少且埋埋频率更低），呈剂量依赖性。5微克剂量的SAR对埋埋行为没有显著影响；它也没有改变ATII对防御性埋埋试验行为反应的抑制作用。这些结果证明，ATII可对介导条件性恐惧相关行为（即防御性埋埋）的中枢递质系统发挥抗焦虑作用。本研究表明，防御性埋埋动物模型是一种相当敏感的试验，符合研究神经肽（如ATII）中枢作用的剂量依赖性敏感性的药理学标准。
