Mazurier S I, Audurier A, Marquet-Van der Mee N, Notermans S, Wernars K
Laboratory of Water and Food Microbiology, National Institute of Public Health and Environmental Protection, Bilthoven, The Netherlands.
Res Microbiol. 1992 Jun;143(5):507-12. doi: 10.1016/0923-2508(92)90097-8.
The analysis of RAPD profiles generated by PCR with a single 10-mer, HLWL74, was compared to bacteriophage susceptibility data for epidemiological typing of Listeria monocytogenes strains. A total of 104 L. monocytogenes strains was screened, all from serogroup 1 or serotype 4b. Of these, 53 had been isolated during 6 different listeriosis outbreaks. The remaining 51 strains were chosen randomly from our collection. A total of 38 RAPD types were observed, although each epidemic group of strains isolated during one of these outbreaks displayed a specific RAPD profile. For 98% of the strains isolated during outbreaks, the correlation between RAPD typing and phage typing was complete. Only one strain, typed as epidemic by phage typing, was clearly distinguishable from the others by RAPD analysis. Among the 51 strains not related to an outbreak, 12 were linked to epidemic groups by RAPD analysis. Two of these rearrangements were supported by phage typing. The remaining 10 strains could be excluded by phage typing from any of the epidemic groups studies. Considering all 104 isolates, the decision to relate a strain to a particular epidemic group or to exclude a strain from any epidemic group was the same for 92 isolates, using either phage typing or RAPD analysis. The RAPD analysis, which is quick, simple and suited for automation, is proposed as an attractive alternative for phage typing in epidemiological studies of listeriosis.
将用单个10聚体HLWL74通过聚合酶链反应（PCR）产生的随机扩增多态性DNA（RAPD）图谱分析结果，与用于单核细胞增生李斯特菌菌株流行病学分型的噬菌体敏感性数据进行比较。共筛选了104株单核细胞增生李斯特菌菌株，均来自血清群1或血清型4b。其中，53株是在6次不同的李斯特菌病暴发期间分离得到的。其余51株是从我们的菌株库中随机选取的。尽管在这些暴发之一期间分离的每个菌株流行组都显示出特定的RAPD图谱，但共观察到38种RAPD类型。对于在暴发期间分离的98%的菌株，RAPD分型与噬菌体分型之间的相关性是完全的。只有一株通过噬菌体分型被鉴定为流行株，但通过RAPD分析可明显与其他菌株区分开来。在与暴发无关的51株菌株中，有12株通过RAPD分析与流行组相关。其中两种重排得到噬菌体分型的支持。其余10株菌株通过噬菌体分型可被排除在任何研究的流行组之外。考虑所有104株分离株，对于92株分离株，使用噬菌体分型或RAPD分析将一株菌株与特定流行组相关联或将其从任何流行组中排除的决定是相同的。RAPD分析快速、简单且适合自动化，被提议作为李斯特菌病流行病学研究中噬菌体分型的一种有吸引力的替代方法。
