PLEDGER R A, POLATNICK J
J Bacteriol. 1962 Mar;83(3):579-83. doi: 10.1128/jb.83.3.579-583.1962.
Pledger, Richard A. (Plum Island Animal Disease Laboratory, Greenport, N. Y.) and Jerome Polatnick. Defined medium for growth of foot-and-mouth disease virus. J. Bacteriol. 83:579-583. 1962.-Foot-and-mouth disease virus, grown in primary bovine calf-kidney cell layers with a defined medium containing glucose as the only organic substrate, produced virus titers equivalent to those obtained with complex media containing serum and lactalbumin hydrolyzate. Mannose was the only other substrate examined that could replace glucose. Krebscycle intermediates did not or only partially supported viral replication. Phosphate was also found essential for reproduction of foot-and-mouth disease virus. The rate of heat inactivation of foot-and-mouth disease virus in defined medium was more than twice that in complex medium. This, however, did not interfere with the usefulness of the defined medium for short-term biochemical investigations.
普莱杰，理查德·A.（纽约州格林波特普拉姆岛动物疾病实验室）和杰罗姆·波拉特尼克。口蹄疫病毒生长的限定培养基。《细菌学杂志》83:579 - 583。1962年。——口蹄疫病毒在初代牛犊肾细胞层中，于含有葡萄糖作为唯一有机底物的限定培养基中生长，产生的病毒滴度与在含有血清和乳白蛋白水解物的复合培养基中获得的滴度相当。甘露糖是所检测的唯一能替代葡萄糖的其他底物。三羧酸循环中间体不能或仅部分支持病毒复制。还发现磷酸盐对口蹄疫病毒的繁殖至关重要。口蹄疫病毒在限定培养基中的热灭活速率是复合培养基中的两倍多。然而，这并不妨碍限定培养基用于短期生化研究。
