Colbert D L
Department of Chemical Pathology, Medical College of St. Bartholomew's Hospital, London, England.
Ther Drug Monit. 1992 Oct;14(5):386-90. doi: 10.1097/00007691-199210000-00008.
Reagents have been developed for the measurement of phenytoin in serum, using the enzyme-multiplied immunoassay approach first introduced by Syva. The assay uses a commercially available antiserum and phenytoin valeric acid coupled to glucose-6-phosphate dehydrogenase as the tracer. It has been adapted for use on an Eppendorf EPOS analyser, which allows 300 assays/h with high precision. Excellent agreement was achieved with commercial reagents, and costs were reduced markedly.
已经开发出了用于测量血清中苯妥英的试剂，采用的是Syva首次推出的酶放大免疫分析方法。该分析使用市售抗血清和与葡萄糖-6-磷酸脱氢酶偶联的苯妥英戊酸作为示踪剂。它已被适配用于Eppendorf EPOS分析仪，该分析仪能够以高精度每小时进行300次分析。与市售试剂取得了极佳的一致性，并且成本显著降低。
