PUCAR Z, KELER-BACOKA M
Science. 1961 Oct 27;134(3487):1369-70. doi: 10.1126/science.134.3487.1369.
When 6-percent colloidal solutions of dextran (molecular weights, 10.000 to 500.000) are mixed with human serum in vitro, a new dextran-lipid fraction appears in paper strip electrophoresis between the starting point and the gamma globulins. The intensity of this dextran-lipid fraction increases with the progressive increase of molecular weight of the dextran used, and this increased intensity of the dextran-lipid fraction is accompanied simultaneously by an appropriate decrease of the lipid fraction migrating with the beta globulins. The intensity of the A-lipoproteins and the neutral fats adsorbed at the starting point seems unaltered by the application of dextran in colloidal solution regardless of the molecular weight of the dextran used. No change of the protein patterns was observed.
当将葡聚糖(分子量为10,000至500,000)的6%胶体溶液与人体血清在体外混合时,在纸条电泳中,起始点与γ球蛋白之间会出现一种新的葡聚糖-脂质组分。该葡聚糖-脂质组分的强度随着所用葡聚糖分子量的逐渐增加而增强,并且这种葡聚糖-脂质组分强度的增加同时伴随着与β球蛋白一起迁移的脂质组分的相应减少。无论所用葡聚糖的分子量如何,胶体溶液中葡聚糖的加入似乎都不会改变吸附在起始点的A-脂蛋白和中性脂肪的强度。未观察到蛋白质图谱有变化。