Tsunetsugu-Yokota Y, Matsuda S, Maekawa M, Saito T, Takemori T, Takebe Y
Department of Immunology, National Institute of Health, Tokyo, Japan.
Virology. 1992 Dec;191(2):960-3. doi: 10.1016/0042-6822(92)90272-q.
In order to study the effect of nef gene expression on viral replication in monocytic cells, we established monocytic (U937 and THP-1) cell transfectants constitutively expressing the human immunodeficiency virus type 1 nef gene. We constructed a plasmid expressing the nef gene derived from an infectious clone, NL432, under the control of SR alpha promoter which can drive a high level of gene expression. We found suppressed viral replication in nef-expressing monocytic cells, although a negative effect of nef was observed, with some variation depending on the virus strain and the cell. We also observed that the expression of the surface CD4 molecule is inversely related to the expression of the nef gene, especially in the U937 transfectants. These results indicate that the suppression of viral replication and the down-modulation of CD4 molecule by nef gene expression occur in monocytic cell lines as in T cell lines.
为了研究nef基因表达对单核细胞中病毒复制的影响，我们构建了持续表达人类免疫缺陷病毒1型nef基因的单核细胞（U937和THP-1）转染细胞系。我们构建了一个质粒，该质粒在SRα启动子的控制下表达源自感染性克隆NL432的nef基因，SRα启动子可驱动高水平的基因表达。我们发现在表达nef的单核细胞中病毒复制受到抑制，尽管观察到nef有负面影响，但根据病毒株和细胞的不同会有一些差异。我们还观察到表面CD4分子的表达与nef基因的表达呈负相关，尤其是在U937转染细胞系中。这些结果表明，nef基因表达对病毒复制的抑制以及CD4分子的下调在单核细胞系中与在T细胞系中一样会发生。
