RODE L J, LEWIS C W, FOSTER J W
J Cell Biol. 1962 Jun;13(3):423-35. doi: 10.1083/jcb.13.3.423.
Resting spores of Bacillus megaterium appear uniformly opaque and undifferentiated under the electron microscope. Germinated spores and spores which have lost their dipicolinic acid underwent characteristic changes in structure. Spores fixed with KMnO(4) lose their dipicolinic acid. Spores fixed with OsO(4) under certain conditions retain their dipicolinic acid. When conventional sectioning procedures are used with either method of fixation, abnormal spore structure is produced as a result of the solution of cellular constitutents. Dry sections of unfixed spores embedded in methacrylate reveal the spore structure in a more normal state. Indirect evidence has been obtained for the existence of a penetration barrier at or near the outer edge of the cortex.
在电子显微镜下,巨大芽孢杆菌的静息孢子看起来均匀不透明且无分化。已萌发的孢子和失去吡啶二羧酸的孢子在结构上发生了特征性变化。用高锰酸钾固定的孢子会失去其吡啶二羧酸。在某些条件下用四氧化锇固定的孢子会保留其吡啶二羧酸。当对两种固定方法都使用常规切片程序时,由于细胞成分的溶解会产生异常的孢子结构。嵌入甲基丙烯酸酯中的未固定孢子的干切片显示出更正常状态的孢子结构。已获得间接证据表明在皮层外边缘或其附近存在渗透屏障。