Koshikawa T, Beaman T C, Pankratz H S, Nakashio S, Corner T R, Gerhardt P
J Bacteriol. 1984 Aug;159(2):624-32. doi: 10.1128/jb.159.2.624-632.1984.
A variant strain that produced spores lacking exosporium was isolated from a culture of Bacillus megaterium QM-B1551. Two additional spore morphotypes were obtained from the parent and variant strains by chemical removal of the complex of coat and outer membrane. Among the four morphotype spores, heat resistance did not correlate with total water content, wet density, refractive index, or dipicolinate or cation content, but did correlate with the volume ratio of protoplast to protoplast plus cortex. The divestment of integument layers exterior to the cortex had little influence on heat resistance. Moreover, the divestment did not change the response of either the parent or the variant spores to various germination-initiating agents, except for making the spores susceptible to germination by lysozyme. The primary permeability barrier to glucose for the intact parent and variant spores was found to be the outer membrane, whereas the barrier for the divested spores was the inner membrane.
从巨大芽孢杆菌QM-B1551的培养物中分离出一种产生缺乏芽孢外壁孢子的变异菌株。通过化学方法去除芽孢衣和外膜复合物，从亲本菌株和变异菌株中获得了另外两种孢子形态型。在这四种形态型孢子中，耐热性与总含水量、湿密度、折射率、吡啶二羧酸或阳离子含量无关，但与原生质体与原生质体加皮层的体积比有关。皮层外部覆盖层的去除对耐热性影响很小。此外，这种去除除了使孢子对溶菌酶萌发敏感外，并没有改变亲本或变异孢子对各种萌发启动剂的反应。完整的亲本孢子和变异孢子对葡萄糖的主要渗透屏障是外膜，而去除覆盖层的孢子的屏障是内膜。
