Nataprawira D S, Harada T, Sekijima A, Mio Y, Terakawa N
Department of Obstetrics and Gynecology, Hasan Sadikin Hospital, Padjadiaran University School of Medicine, Bandung, Indonesia.
Asia Oceania J Obstet Gynaecol. 1992 Sep;18(3):225-30.
We compared the assessment of follicular maturity by follicular diameter or follicular fluid (FF) volume and the subsequent fertilization and cleavage of the oocytes in 36 patients who had ovarian stimulation performed for in vitro fertilization and embryo transfer (IVF-ET). All patients received clomiphene citrate and human menopausal gonadotropin. The daily growth of follicles was assessed ultrasonically for 6 consecutive days before oocyte collection. The follicular diameter was found to be well correlated with the volume of follicular fluid. There were no significant differences among the dominant, second, and third follicles in the rates of oocyte recovery, maturation, fertilization, or cleavage. The rates of oocyte maturation and fertilization in FF volumes of 5.1 to 7.0 ml were significantly higher than in volumes of 1.1 to 5.0 ml, but the cleavage rates were similar. A smaller percentage of embryos developed to the four-cell stage when the oocytes were recovered from follicles with volumes of 1.1 to 3.0 ml. These observations suggest that follicular volume is a good parameter for judging the maturity of oocytes. It is also suggested that, although the oocytes of small follicles can be fertilized and cleaved, oocytes from larger follicles may have a better developmental capacity.
我们比较了通过卵泡直径或卵泡液（FF）体积评估卵泡成熟度，以及随后对36例因体外受精和胚胎移植（IVF-ET）而接受卵巢刺激的患者的卵母细胞进行受精和分裂的情况。所有患者均接受枸橼酸氯米芬和人绝经期促性腺激素治疗。在卵母细胞采集前连续6天通过超声评估卵泡的每日生长情况。发现卵泡直径与卵泡液体积密切相关。优势卵泡、第二卵泡和第三卵泡在卵母细胞回收、成熟、受精或分裂率方面无显著差异。卵泡液体积为5.1至7.0 ml时卵母细胞的成熟和受精率显著高于1.1至5.0 ml时，但分裂率相似。当从体积为1.1至3.0 ml的卵泡中回收卵母细胞时，发育至四细胞期的胚胎比例较小。这些观察结果表明，卵泡体积是判断卵母细胞成熟度的一个良好参数。还表明，尽管小卵泡的卵母细胞可以受精和分裂，但来自较大卵泡的卵母细胞可能具有更好的发育能力。
