Zhang L Z, Zhao W X
Department of Obstetrics and Gynecology, Third Hospital, Beijing Medical University.
Chin Med J (Engl). 1990 Mar;103(3):186-91.
Forty-four infertile women in 44 cycles were stimulated by CC/hMG/hCG (39 cycles) and by CC/hCG (5 cycles) for the purpose of in vitro fertilization (IVF, 37 cases with 35 transfers) and gamete intrafallopian transfer (GIFT, 7 cases). Intraoperative ovum pick-up was performed 32-36 hours after hCG injection. Blood E2 and P levels were determined. Blood E2 was found to be proportional to the number of mature and intermediate oocytes obtained, and correlated with the number of follicles (greater than 10 mm) and the total follicular fluid volume. Altogether 213 follicles aspirated were analyzed. There was a highly significant linear correlation (r = 0.72, P less than 0.01) between the follicular diameter (X cm) on the operation day and the aspirated follicular fluid volume (FFV, Y). The equation of the line was Y = 3.8 X-4.4. The higher concentrations of follicular E2, P, FSH, LH and lower T concentration were crucial to the maturing process of oocytes. It was found that mature oocytes could be retrieved from 26.5% of the small follicles, so it is worthwhile to aspirate the small ones. The fertilization rate was much higher with the mature oocytes than the immature ones. But the morphologically mature oocytes might function differently, i.e. the mature oocytes retrieved from the small follicles or from follicles with unbalanced hormonal microenvironment usually give a low fertilization rate.
44例不孕女性共44个周期，采用克罗米芬/人绝经期促性腺激素/人绒毛膜促性腺激素（39个周期）和克罗米芬/人绒毛膜促性腺激素（5个周期）进行刺激，目的是进行体外受精（IVF，37例，35次移植）和配子输卵管内移植（GIFT，7例）。在注射人绒毛膜促性腺激素后32 - 36小时进行术中取卵。测定血雌二醇（E2）和孕酮（P）水平。发现血E2与获得的成熟及中等大小卵母细胞数量成正比，并与卵泡数量（大于10mm）及卵泡液总体积相关。共分析了213个吸出的卵泡。手术日卵泡直径（X cm）与吸出的卵泡液体积（FFV，Y）之间存在高度显著的线性相关性（r = 0.72，P < 0.01）。直线方程为Y = 3.8X - 4.4。较高浓度的卵泡E2、P、促卵泡生成素（FSH）、促黄体生成素（LH）及较低的睾酮（T）浓度对卵母细胞成熟过程至关重要。发现26.5%的小卵泡可获取成熟卵母细胞，因此抽吸小卵泡是值得的。成熟卵母细胞的受精率远高于未成熟卵母细胞。但形态学上成熟的卵母细胞功能可能不同，即从小卵泡或激素微环境不平衡的卵泡中获取的成熟卵母细胞通常受精率较低。
