Sreedevi P, Sivaramakrishan B, Suresh A, Radhakrishanaiah K
Department of Zoology Sri Krishnadevaraya University, Anantapur, India.
Biomed Environ Sci. 1992 Sep;5(3):208-20.
The levels of soluble, structural and total proteins, and the activities of A1AT and AAT decreased along with an increase in the levels of free amino acids and the activity of protease in the ctenidium, hepatopancreas and foot of the freshwater mussel L. marginalis after 1, 2, 3 and 4 d of exposure to a lethal concentration (115 mg.L-1) of nickel. But the activity of GDH and the level of urea decreased in the hepatopancreas and increased in the ctenidium and foot. A reverse trend was observed in the level of ammonia. In a sublethal concentration (23 mg.L-1), the levels of soluble, structural and total proteins and ammonia decreased in these three organs of the mussel after 1, 5, 10 and 15 d of exposures, with an increase in the levels of free aminoacids, urea and in the activities of protease, A1AT, AAT and GDH. The extent of these changes differed in degree depending on exposure period in the lethal and sublethal concentrations. The results are discussed in order to arrive at the degree of metal stress on the overall nitrogen metabolism of the mussel according to the period of exposure to lethal and sublethal concentrations of nickel.
在暴露于致死浓度（115 mg.L-1）的镍1、2、3和4天后，淡水贻贝L. marginalis的鳃、肝胰腺和足部中，可溶性蛋白、结构蛋白和总蛋白水平以及α1抗胰蛋白酶（A1AT）和抗胰蛋白酶（AAT）的活性随着游离氨基酸水平和蛋白酶活性的增加而降低。但肝胰腺中谷氨酸脱氢酶（GDH）的活性和尿素水平降低，而鳃和足部中则升高。氨水平呈现相反的趋势。在亚致死浓度（23 mg.L-1）下，在暴露1、5、10和15天后，贻贝这三个器官中的可溶性蛋白、结构蛋白和总蛋白水平以及氨水平降低，游离氨基酸、尿素水平以及蛋白酶、A1AT、AAT和GDH的活性增加。这些变化的程度因致死和亚致死浓度下的暴露时间而异。根据暴露于致死和亚致死浓度镍的时间，对结果进行了讨论，以确定金属对贻贝整体氮代谢的胁迫程度。
