Wang H, Ng T B
Department of Microbiology, College of Biological Science, China Agricultural University, Beijing, China.
Biochem Biophys Res Commun. 2001 Nov 9;288(4):765-70. doi: 10.1006/bbrc.2001.5822.
Two novel antifungal peptides, designated alpha- and beta-basrubrins, respectively, were isolated from seeds of the Ceylon spinach Basella rubra. The purification procedure involved saline extraction, (NH(4))(2)SO(4) precipitation, ion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel, ion exchange chromatography on CM-cellulose and FPLC-gel filtration on Superdex peptide column. alpha- and beta-basrubrins exhibited a molecular weight of 4.3 and 5 kDa, respectively. They inhibited translation in a rabbit reticulocyte system with an IC(50) value of 400 and 100 nM, respectively. alpha- and beta-basrubrin inhibited HIV-1 reverse transcriptase by (79.4 +/- 7.8)% and (54.6 +/- 3.6)%, respectively, at a concentration of 400 microM, and (10.56 +/- 0.92)% and (2.12 +/- 0.81)%, respectively, at a concentration of 40 microM. Both alpha- and beta-basrubrins exerted potent antifungal activity toward Botrytis cinerea, Mycosphaerella arachidicola, and Fusarium oxysporum.
从锡兰菠菜（落葵）种子中分离出两种新型抗真菌肽，分别命名为α-和β-紫茉莉素。纯化过程包括盐水提取、硫酸铵沉淀、DEAE-纤维素离子交换色谱、Affi-gel蓝凝胶亲和色谱、CM-纤维素离子交换色谱以及Superdex肽柱FPLC凝胶过滤。α-和β-紫茉莉素的分子量分别为4.3 kDa和5 kDa。它们在兔网织红细胞系统中抑制翻译，IC50值分别为400 nM和100 nM。在400 μM浓度下，α-和β-紫茉莉素分别抑制HIV-1逆转录酶(79.4 ± 7.8)%和(54.6 ± 3.6)%，在40 μM浓度下分别抑制(10.56 ± 0.92)%和(2.12 ± 0.81)%。α-和β-紫茉莉素对灰葡萄孢、花生球腔菌和尖孢镰刀菌均具有较强的抗真菌活性。
