Brauth Steven E, Tang Ye-Zhong, Liang Wenru, Roberts Todd F
Department of Psychology, University of Maryland, College Park, MD 20742, USA.
Brain Res Mol Brain Res. 2003 Sep 10;117(1):97-103. doi: 10.1016/s0169-328x(03)00290-0.
Contact call-driven zenk (zif268, egr1, NGF1A, Krox 24) mRNA expression was mapped with in situ hybridization histochemistry in a vocal learning parrot, the budgerigar (M. undulatus). Relative to controls, call stimulation induced high zenk mRNA expression in all auditory areas including those closely associated with the vocal system within the anterior forebrain (Brauth et al. (2001) J. Comp. Neurol. 432, 481; (2002) Learn. Memory 9, 76). Thus there is a high correspondence between the distributions of neurons exhibiting contact call-driven zenk protein and mRNA expression in budgerigars. Field L2a, an area reported previously to express only perinucleolar zenk protein localization (Brauth et al. (2002) Learn. Memory 9, 76) also showed zenk mRNA expression.
通过原位杂交组织化学技术，在一种能进行发声学习的鹦鹉——虎皮鹦鹉（Melopsittacus undulatus）中，绘制了由接触叫声驱动的即刻早期基因（zenk，即zif268、egr1、NGF1A、Krox 24）mRNA的表达图谱。相对于对照组，叫声刺激在前脑前部所有听觉区域诱导了高表达的zenk mRNA，包括那些与发声系统紧密相关的区域（Brauth等人，(2001)《比较神经学杂志》432, 481；(2002)《学习与记忆》9, 76）。因此，在虎皮鹦鹉中，表现出由接触叫声驱动的zenk蛋白和mRNA表达的神经元分布之间存在高度一致性。之前报道仅表达核仁周围zenk蛋白定位的L2a区（Brauth等人，(2002)《学习与记忆》9, 76）也显示出zenk mRNA的表达。
