Ramakrishna Wusirika, Bennetzen Jeffrey L
Department of Biological Sciences, Purdue University, West Lafayette, IN, USA.
Methods Mol Biol. 2003;236:109-22. doi: 10.1385/1-59259-413-1:109.
Plant genomes show genomic colinearity in spite of the tremendous variability exhibited in their genome size and chromosomal constitution. Comparative genetics can assist in isolation of a mapped gene in a large genome plant species using a small genome plant as a surrogate. Here, we describe various steps involved in the process of gene isolation using genomic colinearity. This involves fine resolution mapping in the large genome species and using common low copy number DNA markers that map to orthologous regions in small and large genome species to isolate candidate genes from the small genome species. Further, alternate strategies are described in cases where the targeted gene is absent in the orthologous region of the small genome species. We also discuss various technologies that can be used for the confirmation of candidate genes.
尽管植物基因组在大小和染色体组成上表现出巨大的变异性，但它们仍显示出基因组共线性。比较遗传学可以利用小基因组植物作为替代物，帮助在大基因组植物物种中分离定位基因。在这里，我们描述了利用基因组共线性进行基因分离过程中涉及的各个步骤。这包括在大基因组物种中进行精细定位，并使用映射到小基因组和大基因组物种直系同源区域的常见低拷贝数DNA标记，从小基因组物种中分离候选基因。此外，还描述了在小基因组物种的直系同源区域中不存在目标基因的情况下的替代策略。我们还讨论了可用于确认候选基因的各种技术。
