新型圆形精子细胞特异性蛋白RSB-66的结晶及初步晶体学分析
Crystallization and preliminary crystallographic analysis of RSB-66, a novel round spermatid-specific protein.
作者信息
Yang Maojun, Li Shentao, Liang Gang, Sun Lei, Zhang Xian, Liu Ning, Pang Hai, Miao Shiying, Wang Linfang, Rao Zihe
机构信息
MOE Protein Science Laboratory and Laboratory of Structural Biology, Department of Biological Science and Biotechnology, Tsinghua University, Beijing 100084, People's Republic of China.
出版信息
Acta Crystallogr D Biol Crystallogr. 2003 Oct;59(Pt 10):1853-5. doi: 10.1107/s090744490301802x. Epub 2003 Sep 19.
Crystals of the RSB-66 protein have been grown at 291 K using NaCl as precipitant. In the refinement of the crystallization this protein, the crystallographic PCR method was used and was found to help in obtaining the best crystals more quickly and easily. The diffraction pattern of the crystal extends to 2.7 A resolution in-house. A full set of X-ray diffraction data were collected to 2.7 A from a single crystal. The crystals belong to space group P4212, with unit-cell parameters a = 90.4, b = 90.4, c = 122.2 A, alpha = beta = gamma = 90 degrees. The presence of two or three molecules per asymmetric unit gives a crystal volume per protein mass (V(M)) of 3.22 or 2.14 A(3) Da(-1), respectively.
已使用氯化钠作为沉淀剂在291 K下培养出RSB - 66蛋白晶体。在该蛋白晶体的优化过程中，采用了晶体学PCR方法，发现该方法有助于更快、更轻松地获得最佳晶体。该晶体的衍射图谱在内部可延伸至2.7 Å分辨率。从单个晶体收集了一套完整的X射线衍射数据，分辨率为2.7 Å。这些晶体属于空间群P4212，晶胞参数为a = 90.4，b = 90.4，c = 122.2 Å，α = β = γ = 90°。每个不对称单元存在两个或三个分子时，每蛋白质量的晶体体积（V(M)）分别为3.22或2.14 ų Da⁻¹。
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