Nielsen Line, Andersen Mads Klindt, Jensen Tove Dannemann, Blixenkrone-Møller Merete, Bolt Gert
Laboratory of Virology and Immunology, Royal Veterinary and Agricultural University, Copenhagen, Denmark.
Virus Genes. 2003 Oct;27(2):157-62. doi: 10.1023/a:1025724526378.
We examined the consequences of isolation and adaptation to Vero cells for the receptorbinding haemagglutinin (H) gene of four syncytia-forming isolates of canine distemper virus (CDV) and of a dolphin morbillivirus isolate. A Vero-adapted CDV isolate exhibited biased hypermutation, since 11 out of 12 nucleotide differences to other isolates from the same epidemic were U-C transitions. Most of these transitions appeared to have taken place during in vitro cultivation. Previously, biased hypermutation in morbilliviruses has almost exclusively been described for subacute sclerosing panencephalitis and measles inclusion body encephalitis, which are rare measles virus brain infections. Amino acid changes in the H proteins were not required for Vero cell adaptation, suggesting that Vero cells express receptors for wild-type morbilliviruses. This strongly indicate the existence of other morbillivirus receptors than CD46 and CDw150.
我们研究了犬瘟热病毒（CDV）的四种形成合胞体的分离株以及一种海豚麻疹病毒分离株的受体结合血凝素（H）基因在适应Vero细胞及分离过程中的变化情况。一株适应Vero细胞的CDV分离株表现出偏向性超突变，因为与同一流行毒株的其他分离株相比，12个核苷酸差异中有11个是U-C转换。这些转换大多似乎发生在体外培养过程中。此前，偏向性超突变几乎仅在亚急性硬化性全脑炎和麻疹包涵体脑炎中被描述过，这两种都是罕见的麻疹病毒脑部感染。Vero细胞适应过程中H蛋白的氨基酸变化并非必需，这表明Vero细胞表达野生型麻疹病毒的受体。这有力地表明，除了CD46和CDw150之外，还存在其他麻疹病毒受体。
